賀建超; 石國昌
陜西省微生物研究所; 陜西省微生物研究所 西安; 西安
【中文摘要】 本文報道了使用蝸牛酶和纖維素酶從糙皮側耳和佛羅里達側耳雙核菌絲體中,成功地分離了高產量的原生質體。菌絲體培養時間,混合酶液 pH 值,一定濃度的酶液用量,酶解溫度和時間與原生質體形成相關。原生質體的兩種再生型被觀察到,并計算了再生頻率,糙皮側耳為2.5%.佛羅里達側耳為2.3%。
【英文摘要】 The formation and regeneration of mycelial protoplasts from Pleu- rotus ostreatus and P.florida using snailase and cellulase were studied.The amount of release of protoplasts is related to mycelial age,buffer pH,temperature and dose of enzyme used.To achieve a better enzymolysis result,young mycelia, botained from the edge of colonies which were incubated on solid plates at 25℃ for 4 days,were used in this study.5ml 1% of snailase and cellulase was added to one gram mycelia (wet weight) in pH5.8 buffer for enzymolysis at 35℃ for 4.5 hours.The regeneration frequeucy of protoplasts of both strains were observed by using RM medium,2.5% for Pleurotus ostreatus and 2.3% for P.florida. Two regeneration types were obtained in LRM medium.For the first type,proto- plasts directly produced one or more germ tubes; but for the second,protoplasts produced bud-like structures at beginning and then develo ped into germ tubes.
【中文關鍵詞】 原生質體; 再生型; 糙皮側耳; 佛羅里達側耳; 雙核菌絲體
【英文關鍵詞】 protoplasts; regeneration type; Pleurotus ostreatus; P.florida; two nuclear mycelia
【文獻出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,1989年05期 【DOI】CNKI:SUN:ZSYJ.0.1989-05-006