方春暉; 林志彬
北京大學醫學部藥理學系; 北京大學醫學部藥理學系 北京100083 ; 北京100083
【中文摘要】 本文初步考察巴氏蘑菇提取物 (ABE)對于小鼠離體脾淋巴細胞功能的影響。采用的方法包括正常和免疫受抑情況下脾淋巴細胞對于刀豆蛋白A刺激的增殖反應和單雙向混合淋巴細胞反應。結果發現 ,在 5～ 10 0 μg/ml濃度范圍 ,ABE對小鼠離體脾淋巴細胞的增殖反應具有增強作用 ;在 5～ 5 0 μg/ml濃度范圍 ,ABE可以部分恢復免疫受抑的小鼠離體脾淋巴細胞對刀豆蛋白A的應答能力 ;在 10～ 5 0 0 μg/ml范圍內 ,ABE對單向和雙向混合淋巴細胞反應都具有不同程度的增強作用 ,更高的濃度則具有抑制作用
【英文摘要】 We conducted a series of experiments to observe the effect of Agaricus blazei Murill.extract(ABE)on the functions of murine lymphocytes in vitro.The methods include splenocyte proliferation induced by ConA,using both normal murine lymphocytes and lymphocytes inhibited by cyclosporin A or hydrocortisone.mixed lymphocyte reaction was conducted using normal C57BL/6j lymphocytes and C57BL/6j lymphocytes pre-incubated with mitomycin C.We found that ABE(5～100μg/ml)can enhance the proliferation of murine lymphocytes stimulated by ConA in vitro,and can partially restore the response of inhabited lymphocyte to ConA within 5～50μg/ml.ABE(10～500μg/ml)cna also enhance mixed lymphocyte reaction.
【中文關鍵詞】 巴氏蘑菇; 淋巴細胞; 免疫藥理學
【英文關鍵詞】 Agaricus blazei Murill; Lymphocytes; Immunopharmacology
【文獻出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,2002年03期 【DOI】CNKI:SUN:ZSYJ.0.2002-03-018