胡樂琴; 潘迎捷
上海水產大學生命與技術學院; 上海水產大學食品學院 上海200090; 上海200090
【中文摘要】 概述了食用菌轉化取得的進展和存在的問題,詳細介紹了篩選標記、啟動子、轉化方法、整合方式以及將來的研究方向等方面的研究狀況。篩選標記主要有營養缺陷型、藥物抗性標記;啟動子主要是同源啟動子ras基因和gpd基因啟動子;轉化方法主要有PEG法、電擊法、限制性內切酶介導法、農桿菌介導法,對比分析了各種方法的優缺點;重組方式主要是多位點整合;甲基化是導致轉錄水平低的一個原因;可能的提高轉化率措施有采用強啟動子、加入同源片段進行同源整合。
【英文摘要】 The progress and the problems in the transformation of edible fungi were summarized after introducing the select marker,the promoter,the ways of transformation,the pattern of integration and the future direction of the research in detail.Two main select markers were auxotroph and drug resistance.The promoter were major the ras gene of homologous promoter and the gpd gene promoter;the methods of transformation were main PEG method,electroporation method,restrictive enzyme-mediatedintegration and Agrobacterium-mediated method,which were advantages and disadvantages respectively after compared analyzing.The style of recombination is integration on multiple locus.The heavy methylation of the integrated DNA was one reason of low transcript.The possible measures which improved the frequency of transformation made homologous recombination by using strong promoter and adding homologous sequence.
【中文關鍵詞】 食用菌; 轉化; 育種; 分子技術
【英文關鍵詞】 Edible fungi; Transformation; Breeding; Molecular-technology
【基金】上海市農委重點攻關項目資助
【文獻出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,2006年04期 【DOI】CNKI:SUN:ZSYJ.0.2006-04-001