馬金寶; 沈業壽; 李峰; 史新強; 趙根海; 解要敏
安徽大學生命科學學院生化微生物研究所安徽省生態工程與生物技術重點實驗室
【中文摘要】 采用鄰苯三酚自氧化法、Fenton反應體系法、DPPH體系法,以抗壞血酸為陽性對照,對亮菌多糖-1b(ATPS-1b)清除超氧陰離子自由基(O2-·)、羥自由基(·OH)和二苯代苦味肼基自由基(DPPH·)的效果進行了測定。結果表明ATPS-1b對O2-·、·OH和DPPH·3種自由基均具有很強的清除作用,且存在良好的量效關系,隨著ATPS-1b濃度的增大,其清除效果增強;清除O2-·、·OH、DPPH·3種自由基的EC50值分別為0.69mg·mL-1、8.73mg·mL-1、2.01mg·mL-1;對3種自由基的最大清除率分別為86.17%、76.4%、78.52%;ATPS-1b具有顯著的體外抗氧化活性。
【英文摘要】 The effects of polysaccharide-1b from Armillariella tabescens(ATPS-1b) to remove superoxide anion free radical,hydroxyl free radical and DPPH· free radical were determined by the methods of the auto-oxidation of pyrogallol system,Fenton reaction system and DPPH· system. The results showed that ATPS-1b had strong scavenging capacity to O2-·,·OH and DPPH·. Furthermore,scavenging capacity and anthocyanins concentration showed a position of dose-response relationship. The three free radicals had strong scavenging results with the increasing of concenteation. The EC50 of scavenging free radicals were 0.69 mg·mL-1,8.73 mg·mL-1,2.01 mg·mL-1respectively and maximum scavenging rates were 86.17%,76.4%,78.52%. ATPS-1b had significant antioxidant activity in vitro experiments.
【中文關鍵詞】 亮菌; 多糖; 超氧陰離子自由基; 羥自由基; 二苯代苦味肼基自由基; 抗氧化活性
【英文關鍵詞】 Armillariella tabescens; Polysaccharide; Superoxide anion free radical; Hydroxyl free radical; DPPH· free radical; Antioxidant activity
【文獻出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,2008年06期 【DOI】CNKI:SUN:ZSYJ.0.2008-06-018