陳瑞鵬; 賈小寧; 郭立忠
青島農業大學生命科學學院山東省應用真菌重點實驗室
【中文摘要】 采用RAPD、SRAP兩種標記方法分別對6個繡球菌(Sparassis crispa)菌株進行親緣關系分析,結果分析表明2種方法結果一致,平均每條引物組合產生的條帶數分別為20.5和24.5,多態性條帶的比例分別為91.4%和78.8%。結果顯示,6個菌株間均有遺傳上的差異,菌株4號、5號同源性最大,相似系數分別達到了0.981和0.875,說明菌株4號、5號可能是同物異名。
【英文摘要】 Six strains of Sparassis crispa conserved by Agricultural Applied Mycology Lab, Qingdao Agricultural University, were studied by using the molecular techniques of RAPD and SRAP in this experiment. The results showed that the results of two methods are consistent. Each primer combination generated the average strip of 20.5 and 24.5, respectively. The proportion of polymorphic bands were 91.4% and 78.8% respectively, and 6 strains were different at genetic. Sparassis crispa strain 4 and 5 exhibited a higher genetic similarity (similar coefficients were up to 0.9813 and 0.8570), which showed close phylogenetic relationship.
【中文關鍵詞】 繡球菌; RAPD; SRAP; 聚類分析
【英文關鍵詞】 Sparassis crispa; RAPD; SRAP; Cluster analysis
【基金】國家支撐項目,黃淮平原區農田秸稈資源綜合利用技術集成研究與示范(2007BAD89B09-15)
【文獻出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,2010年02期 【DOI】CNKI:SUN:ZSYJ.0.2010-02-015