許曉燕; 鄭林用; 李艷; 江南; 余夢瑤; 羅霞
四川省中醫藥科學院中藥細胞與分子生物學實驗室; 四川省農業科學院; 成都市經濟信息中心
【中文摘要】 采用雙向固體發酵工程技術,以靈芝為發酵菌株,在含有不同比例丹參邊角料的培養基中進行發酵,觀察了不同培養基中菌絲的生長情況,測定了發酵產物(靈丹菌質)中多糖、蛋白質、三萜、丹參酮的含量變化,并研究了其提取物對小鼠活血化瘀功效的影響。結果表明靈丹菌質的最佳發酵組合為組合4,發酵40d,多糖含量26.48μg·g-1,蛋白質含量為397.24μg·g-1,靈芝酸三萜含量為4.92%。藥效學實驗顯示,靈丹菌質組合4具有活血化瘀的藥效,優于丹參藥材。
【英文摘要】 With two-way pattern solid-state fermentation technique, Ganoderma lucidum was fermented in the drug-containing medium containing different proportion of Salvia miltiorrhiza bunge by-product. Hyphal growth of G. lucidum was surveyed. The contents of polysaccharide, water-solubility protein, triterpene and tanshinton of solid-fermented products (SFP) were tested. In addition, the effect of activiting blood circulation to dissipate blood stasis on mouse fed with extracts was evaluated. The results showed that formulation 4 was the optimal medium, of which fermentation period was 40 d. Polysaccharide, crude protein, Ganoderma tanshinton of optimal SFP were 26.48 mg·g-1, 397.24 μg·g-1and 4.92%, respectively. From the results of pharmaco-dynamical experiments, the effects of SFP were better than that of Salvia miltiorrhiza.
【中文關鍵詞】 靈芝; 丹參邊角料; 雙向發酵; 藥效品質
【英文關鍵詞】 Ganoderma lucidum; Salvia miltiorrhiza bunge by-product; Two-way pattern solid-state fermentation; Pharmacody- namic action
【基金】省中醫管理局項目;; 省應用基礎項目(2008JY0006-2)
【文獻出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,2010年02期 【DOI】CNKI:SUN:ZSYJ.0.2010-02-013