詹才新; 凌霞芬; 楊家林
上海市農業科學院食用菌研究所農業部食用菌遺傳育種重點開放實驗室; 上海市農業科學院食用菌研究所; 鄂州市衛生?？茖W校 上海201106 ; 上海201106 ; 鄂州436000
【中文摘要】 以傳統的形態 ,生理生化分析和最新的DCS -PDMA性親和性測定方法為基礎 ,結合群體分離分析和RAPD技術對來源于同一雙孢蘑菇異核體菌株的 12個不育同核原生質體個體進行分析 ,篩選與性親和性相關的分子標記。研究結果表明 ,供試的 12個不育同核原生質體個體被分成兩大類性親和性類型 ,其中一類 (A+ )包括不育同核原生質體個體B、C、D、E、F、G、H、I、J、L ,另一類 (A-)則僅僅包括不育同核原生質體個體K和M ,同時篩選到一個與性親和性相關的分子標記OPA16 150 0 。從而為間接地利用雙孢蘑菇本身特有的交配型作標記來指導雜交育種工作和進一步將其性親和性基因定位分離克隆奠定了堅實的基礎。
【英文摘要】 sterile homokaryotic protoplast isolates from the same heterokaryotic strain of Agaricus bisporus were analyzed in this paper. A molecular marker OPA16 1500 related to sexual compatibility was found using bulk segregation analysis and RAPD technology combined with the newest DCS-PDMA method and the traditional analysis of the morphological, physiological and biochemical characteristics of Agaricus bisporus. The results indicated that 12 sterile homokarytic protoplast isolates tested could be divided into two groups:group A + included B, C, D, E, F, G, H, I, J, L; group A - was made of K and M. Therefore, a solid foundation was established for Agaricus bisporus breeding through indirectly using its mating-type as marker for further location, isolation and cloning of its sexual compatibility gene.
【中文關鍵詞】 雙孢蘑菇; DCS-PDMA方法; 群體分離分析; 性親和性相關分子標記; 雜交育種
【英文關鍵詞】 Agaricus bisporus; DCS PDMA method; Bulk segregation analysis; Molecular marker of sexual compatibility; Cross breeding
【基金】國家自然科學基金項目 (95 - 0 1- 0 4 )
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2002年04期 【DOI】CNKI:SUN:SYJB.0.2002-04-000