LI Haibo1; WU Xueqian1; 2; YING Guohua3; WEI Hailong1; 2; FU Lizhong1; 2; WU Qingqi1; 2; XU Lin1; LIU Qing21Biotechnology Institute; Zhejiang Academy of Forestry Science; Hangzhou; Zhejiang 310023; China; 2Lishui Edible Fungi Research and Development Center; Zhejiang Essence Fungi Development Co.; Ltd; Lishui; Zhejiang 323000; China; 3Lishui Academy of Forestry Science; Lishui; Zhejiang 323000; China
【英文摘要】 RAPD molecular markers and ITS sequence comparisons were used to establish the relationship between Boletus appendiculatus fruit bodies collected from Qingyuan, Zhejiang Province, and mycelium isolated from the fruit body tissue. RAPD analysis of extracted genomic DNA revealed that B. appendiculatus fruit bodies (fb) and the isolated fungal mycelium (mb) formed a single cluster with a Dice coefficient value of 0.92. Comparison of ITS sequences from these samples showed that base sequences and fragment lengths were identical, thereby confirming that the fungal mycelium isolated from B. appendiculatus fruit bodies was the vegetative form of the mushroom. Our data suggest that RAPD fingerprinting combined with ITS sequence comparisons constitute an effective and feasible method for identifying mycelium isolated from ectomycorrhizal fungi.
【英文關鍵詞】 Boletus appendiculatus; Identification; Molecular marker; Pure culture
【基金】Supported by the Foundation of Zhejiang Province ( Nos 2004C22032, Y304403) and the Foundation of LishuiCity (No 2001003)
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2007年01期 【DOI】CNKI:SUN:SYJB.0.2007-01-001