陽飛; 張華山; 董殿波; 顧亞婧
湖北工業大學生物工程學院
【中文摘要】 本文對美味牛肝菌(Boletus edulis)液體發酵生產粗多糖的培養基和發酵條件進行了篩選。在實驗范圍內,美味牛肝菌發酵生產粗多糖的最適培養基配方為:馬鈴薯10.0%,蔗糖2.0%,蛋白胨0.6%,KH2PO40.3%,MgSO4.7H2O0.2%,CaCO30.2%,ZnSO4.7H2O0.1%。在初始pH5.05.4,振蕩轉速120r/min,培養溫度2528℃,250mL三角瓶中裝液量40%,加10顆玻璃珠,發酵時間5d時搖瓶培養粗多糖產量最高,達3.161g/L。經10L機械攪拌發酵罐放大動態實驗,研究pH值、溶氧、菌絲生物量(濕重)以及粗多糖產量的變化,可知最適發酵時間為96h。
【英文摘要】 The effects of selected culture parameters on polysaccharide production by a strain of Boletus edulis are described.Optimal conditions for small-scale polysaccharide production in submerged flask cultures were as follows:a fermentation medium consisting of 10% potato,2% sucrose,0.6% peptone,0.10% ZnSO4·7H2O,0.2% CaCO3,0.3% KH2PO4 and 0.2% MgSO4·7H2O,pH 5.0～5.4,120 r/min shaking speed,temperature 25～28 ℃,40% medium loading ratio,10 glass beads per flask,and a 5-day incubation period.Under these conditions,polysaccharide production reached a peak of 3.161 g/L.In 10-L fermenter cultures using the same medium,pH,temperature,a 50% medium loading ratio,a similar stirrer speed,and an aeration rate of 0.8 vvm,highest polysaccharide yields were obtained after four days.
【中文關鍵詞】 美味牛肝菌; 發酵條件; 搖瓶培養; 發酵罐
【英文關鍵詞】 Boletus edulis; culture parameters; submerged flask culture; fermenter
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2009年01期 【DOI】CNKI:SUN:SYJB.0.2009-01-018