金針菇工廠化生產中黑腐病病原菌的分離與鑒定Isolation and Identification of a Bacterium Causing Black-rot Disease of Factory-Grown Flammulina velutipes
【作者】 黃春燕; 張柏松; 萬魯長; 張海蘭; 郭惠東; 單洪濤; 鄭繼發;
【Author】 HUANG Chunyan1,ZHANG Baisong1,WAN Luzhang1,ZHANG Hailan1,GUO Huidong1,SHAN Hongtao1,ZHENG Jifa2(1Agricultural Resources and Environment Institute,Shandong Academy of Agricultural Sciences,Jinan,Shandong 250100,China;2College of Plant Protection,Shandong Agricultural University,Taian,Shandong 271018,China)
【機構】 山東省農業科學院農業資源與環境研究所; 山東農業大學植保學院;
【摘要】 從山東省金針菇(Flammulina velutipes)工廠化生產中發現一種新病害,將其病原菌進行分離純化,并經柯赫氏法則驗證,該病原菌為致病菌。根據病原菌的形態特征、培養性狀、生理生化及16SrDNA序列分析,鑒定其為托拉氏假單胞菌(Pseudomonas tolaasii)。
【Abstract】 A bacterium isolated from black-rot disease-affected Flammulina velutipes fruit bodies grown in a mushroom cultivation facility in Shandong Province,China,was identified as Pseudomonas tolaasii on the basis of morphological and culture characteristics,physiological and biochemical reactions,and 16S rDNA gene sequence analysis.Application of Koch’s Postulates confirmed the bacterium as the causative agent of the disease.
【關鍵詞】 金針菇; 黑腐??; 托拉氏假單胞菌;
【Key words】 Flammulina velutipes; black-rot disease; Pseudomonas tolaasii;
【基金】 山東省科技發展計劃金針菇工廠化生產重要病害安全防控技術研究[編號:2010GNC10909]的部分研究內容
【分類號】S436.461