一種不經熱浴從雙孢蘑菇子實體中提取DNA的新方法(英文)
A New Protocol for Extracting DNA from Agaricus bisporus Fruit Bodies without Thermal Incubation
【作者】 姬宏超； 萬艷娟； 莫美華； 徐學鋒；
【Author】 JI Hongchao,WAN Yanjuan,MO Meihua,XU XuefengCollege of Food Science,South China Agricultural University,Guangzhou,Guangdong 510642,China
【機構】 華南農業大學食品學院；
【摘要】 介紹一種不經熱浴快速從雙孢蘑菇子實體中提取DNA的新方法,并與傳統的CTAB和SDS法相比較,結果顯示:該方法得到的DNA完整性好,時間短,步驟少,有毒試劑用量少,在提取DNA的質量、得率、時間及完整性等方面都優于傳統的CTAB和SDS法,為食藥用菌子實體DNA的提取參考。
【Abstract】 A new,simple and rapid method for obtaining DNA from Agaricus bisporus fruit bodies is described and compared with the widely used CTAB and SDS methods in terms of yield,DNA quality and integrity,and duration of extraction time.Total DNA quality was estimated by restriction endonuclease digestion and by ITS,ISSR and RAPD analyses using standard protocols.Our data demonstrated that the new method renders improved yields of total DNA of high quality and integrity,and is less time-consuming than ex...
【關鍵詞】 雙孢蘑菇； DNA提??； 內切酶消解；
【Key words】 Agaricus bisporus； DNA isolation； restriction endonuclease digestion；
【基金】 Supported by National Natural Science Foundation of China(No.30800774)
【分類號】S646