李玉祥; 周瀅; 李惠君
南京農業大學; 南京農業大學; 南京農業大學 江蘇南京 210095; 江蘇南京 210095; 江蘇南京 210095
【中文摘要】 TritonX一100提取香菇單核體L52-adc“和金針菇單核體Fv23的菌體壁抗原,分別注射兔子制備抗血清。同樣方法制備L52-adc“和Fv23原生質體融合子F02,F03的菌體壁抗原,瓊脂糖雙向擴散顯示融合子F02、F03的菌體壁抗原能與所制備的親本抗血清發生特異性沉淀反應。表明兩個融合親本的菌體壁抗原基因在融合子中得到表達。從而也進一步證實融合子確為兩親本的雜合子。
【英文摘要】 Mycelial wall antigens were abstracted with TritonX-100 from the mycelium of Lenti- nus edodes monocaryon L52-ade and Flammulina velutipes monocaryon Fv23 and used for immunonizing rabbits to prepare antisera. The immunological reaction between antigens and antisera was tested with immunodif- fusion on agarose gel. The results show that the mycelial wall antigens of the fusants react specifically with the antisera and common pricipitin bands were formed on the gel. This indicates that the mycelia wall anti- gen genes of two parental strains have been expressed in the fusants and therefor confirms the fusants as hy- brids of the two parental strains.
【中文關鍵詞】 免疫鑒定; 原生質體融合; 菌體壁抗原
【英文關鍵詞】 Immunoidentification; Immunodiffusion; Protoplast fusant; Mycelial wall antigen; Basidiomycetes
【文獻出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,1997年02期 【DOI】CNKI:SUN:ZSYJ.0.1997-02-005