孫培龍; 趙明珠
浙江工業大學輕工系; 浙江工業大學輕工系 杭州 310014; 杭州 310014
【中文摘要】 本文報道了一種以紙電泳分析香菇嘌呤的方法,該法能很好地分離各種核苷酸干擾物,回收率為89％,相對誤差5％。本文重點報導了從香菇柄中提取香菇嘌呤的方法,研究證實菇柄按1:8～10的固液比加75％(v/v)的乙醇在自然狀況下浸泡6hr,搗至2mm×5mm的漿狀,于83～85℃水浴中回流萃取2hr,香菇漂呤的提取得率可達161mg％,比資料報道的70mg％高2.3倍。
【英文摘要】 A analytical method for eritadenine using paper electrophoresis was reported. The interfer- ences of the vary nucleotides could be seperated. The rate of recovery of this method was 89%, and the rela- tive error was 5%. A extracting method of eritadenine from the stem of Lentinus edodes was also reported. The better technique of extraction was proofed as follows : A portion of the stem of L. edodes was naturally soaked with 810 portion of 75% (v/v) alcohol for 6 hours, then was pounded to small pieces which the size was 2mm× 5mm. After that, the pulp was extracted for 2 hours at 8385℃. The rate of extraction of eritadinine was 161 mg%, it was 2.3 times as much as 70mg% that was reported by the reference.
【中文關鍵詞】 香菇嘌呤; 提取; 分析測定
【英文關鍵詞】 eritadenine; extraction; analysis; determination
【基金】浙江省科委項目
【文獻出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,1997年02期 【DOI】CNKI:SUN:ZSYJ.0.1997-02-015