尚德靜; 王關林; 方宏筠
遼寧師范大學食品生化研究所; 遼寧大連生物工程研究所!遼寧大連116029; 遼寧大連生物工程研究所!遼寧大連116029
【中文摘要】 本文探討了靈芝 (Ganodermalucidum)菌絲原生質體的制備及再生的條件。實驗結果表明 :采用MYG為靈芝菌絲培養基 ,2 %真菌溶壁酶 (Lywallzyme)作脫壁酶 ,0 6mol/L甘露醇作滲透壓穩定劑 ;對培養 6d的靈芝菌絲在 30℃下進行酶解 ,酶解時間 4- 6h ,可產生約 3 0× 1 0 6個 /mL (0 1g菌絲 )原生質體 ,并在添加靈芝子實體浸出汁的雙層培養基上實現了靈芝菌絲原生質體的再生。
【英文摘要】 The conditions of protoplast formation and regeneration from the mycelia of Ganoderma lucidum were studied.Experimental results indicated that 3 0×10 6/mL protoplast of the mycelia of Ganoderma lucidum was obtained under optimal conditions,which are described as follows:Using 0 6mol/L mannitol as osmotic stabilizer;2% Lywallzyme as the enzyme digesting the cell wall;Using 6 days old MYG liquid cultures of the mycelia Ganoderma lucidum;at 30℃,enzyme digesting 4～6 hours.When incubated on two layer plates of agar,the protoplasts regenerated and formed new mycelia of Ganoderma lucidum.
【中文關鍵詞】 靈芝菌絲; 原生質體
【英文關鍵詞】 Mycelia of Ganoderma lucidum; Protoplasts
【文獻出處】 中國食用菌,EDIBLE FUNGI OF CHINA,編輯部郵箱,2000年02期 【DOI】CNKI:SUN:ZSYJ.0.2000-02-000