羅信昌
華中農業大學!湖北武漢430070
【中文摘要】 按照植物病理學原則 ,蘑菇分離物應依據柯赫法則 (Koch’spostulate)回接。在一定條件下 ,人工誘發子實體得以鑒別。但是一些蘑菇種類 (如某些共生食用蘑菇 )難以人工誘發子實體 ,即使能形成子實體也費時費力。本文介紹DNA指紋鑒別技術 ,即運用RAPD -PCR技術制成分離物及對應子實體指紋圖譜 ,分析它們間DNA同源性及遺傳相似度以鑒別分離物真偽 ,探求快速 ;簡便 ;有效的鑒別方法。
【英文摘要】 The isolate of mushrooms should be identified by back inoculation,artificial induce sporophore formation under adapted condition according to Koch's postulate. However,it is very difficult to form sporophore by artificial inducing in some species,i.g.ectomycorrhizal mushrooms.Even if sporophore formation,it would take a long time and lots of efforts.Isolate would be identified by application PAPD-PCR technique,to make DNA band,analysis DNA homogeneity,DNA similarity coefficient between isolate and sporophore,which is of speed,handy and availble for characterization isolate of mushroom.
【中文關鍵詞】 蘑菇; 分離物; RAPD-PCR; DAN圖譜
【英文關鍵詞】 Mushrooms; Isolate; RAPD-PCR; DNA band
【基金】國家自然科學基金!39770 0 2 5 ;; 高等學校博士學科點專項基金資助項目部分內容
【文獻出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,2001年03期 【DOI】CNKI:SUN:ZSYJ.0.2001-03-000