虞磊; 沈業壽; 李賡; 吳建民; 謝群英
安徽大學生命科學學院糖復合物與糖工程實驗室; 安徽省生態工程與生物技術重點實驗室; 安徽大學生命科學學院糖復合物與糖工程實驗室; 安徽大學生命科學學院糖復合物與糖工程實驗室; 安徽大學生命科學學院糖復合物與糖工程實驗室 合肥230039; 合肥230039; 合肥230039; 合肥230039; 合肥230039
【中文摘要】 對蜜環菌在不同液態培養方式下胞內多糖的合成、組成以及抗眩暈癥活性進行比較。結果顯示,蜜環菌在搖床培養條件下形成菌絲球,菌絲多糖(AMP)和生物量在第8d達到最高產量,分別為124.32mg/L和10.85g/L,蜜環菌在靜置培養條件下形成菌索,菌索多糖(ARP)與生物量在20d時達到最高,分別為588.40mg/L和37.17g/L,靜置培養對蜜環菌胞內多糖的合成有利;兩種多糖在多糖含量、蛋白質含量、糖醛酸含量以及皂苷含量等組成成分上基本一致;AKTA-purifier快速層析純化系統(FPLC)檢測顯示,AMP與ARP有四個相同的多糖組分,ARP另多出一個組分;抗機械旋轉所致小鼠眩暈癥試驗表明AMP與ARP活性相似,均能顯著縮短眩暈小鼠逃避電擊所用的時間(P<0.01),增加小鼠眩暈后的進食量,因而對機械旋轉所致眩暈癥具有一定療效。
【英文摘要】 To study the effects of intrapolysaccharides production and component from Armillaria mellea in different liquid culturing methods and anti-vertigo function.Results showed that,under submerged cultivation,AMP(polysaccharides from mycelium of Armillaria mellea) and biomass achieves the maximum production rate in 8th days,were 124.32mg/L and 10.85 g/L respectively.ARP(polysaccharides from rhizomorph of Armillaria mellea) and biomass reached 588.40mg/L and 37.17g/L,at the time of 20 days.Quiet cultivation is better of intrapolysaccharides production for Armillaria mellea.Two intrapolysaccharides have close composition in polysaccharide content,protein content,uronic acid content,and saponin content;FPLC showed that AMP and ARP have four similar polysaccharides components,ARP also have another component;The experiment of opposing function of vertigo caused by machinery rotate to mice showed that AMP and ARP can obviously shorten the escaping time of electrical shock(P<0.01),and obviousl...更多y increase food intake in mice,so the intrapolysaccharides of Armillaria mellea have anti-vertigo function. 還原
【中文關鍵詞】 搖床培養; 靜置培養; 胞內多糖; FPLC; 抗眩暈
【英文關鍵詞】 submerged cultivation; quiet cultivation; intrapolysaccharides; FPLC; anti-vertigo
【基金】國家自然科學基金(3040066);; 安徽省教育廳自然科學基金(2003kj021)
【文獻出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,2006年02期 【DOI】CNKI:SUN:ZSYJ.0.2006-02-014