李祝; 肖洋; 梁宗琦
貴州大學生命科學院; 貴州省產品質量監測院; 貴州大學生命科學院 貴陽550025; 貴陽550025; 貴陽550025
【中文摘要】 從疫霉Phytophthorasp.(YL)和曲霉As-pergillussp.(P6-1)兩株真菌菌絲體中分別提取其多糖作為激發子,在Cordycepsmillitaris97-0706菌株的不同生長階段加入,觀察不同多糖激發子對97-0706菌株蟲草菌素含量的影響。結果表明:當YL多糖單獨加入時,僅對蟲草菌素含量有影響,而單獨加入P6-1多糖,也僅對生物量有影響。但在發酵0h,加入60μg/mLYL多糖,當發酵到72h,再加入80μg/mLP6-1多糖,繼續發酵120h,蟲草菌素的含量可達到21.98±0.14mg/g(P<0.01),為對照的5.7倍,生物量可達到35.89±0.32g/l(P<0.01),比對照提高1.49倍。
【英文摘要】 The polysaccharide was extracted respectively from the mycelium of Phytophthora sp.(YL) or Aspergillus sp.(P6-1),and was used as elicitor at different mycelial growth phases of Cordyceps millitaris 97-0706 to observe the effect of the content for Cordycepin because there were different polysaccharide elicitors.The results showed that when only YL polysaccharide was added to it,it could only affect the content of Cordycepin,and when P6-1 polysaccharide was added to it,it only could affect the biomass.But when it fermented at 0h,60ug/ml YL polysaccharide was first added to it.Then 80ug/ml P6-1 polysaccharide was added to it after fermenting 72h.When it fermented up to 120h,the content of Cordycepin reached to 21.98±0.14mg/g(P<0.01),which was 5.7 times of CK,and biomass could reach to 35.89±0.32g/l(P<0.01),which was 1.49 times of CK.
【中文關鍵詞】 蟲草菌素; 真菌多糖; 激發子; 蛹蟲草; 生物量
【英文關鍵詞】 Cordycepin; Fungal Polysaccharide; Elicitor; Cordyceps millitaris
【文獻出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,2006年03期 【DOI】CNKI:SUN:ZSYJ.0.2006-03-012