黃慧艷; 張曉昱; 孫永林
湖北華中科技大學生命科學與技術學院; 湖北華中科技大學生命科學與技術學院; 湖北華中科技大學生命科學與技術學院 武漢430074; 武漢430074; 武漢430074
【中文摘要】 選取3株不同種屬白腐菌(K1靈芝屬Ganoderma lucidum,B2側耳屬Pleurots sp.,A3香菇屬Lentinula edodes),2種不同木質纖維素基質(玉米秸粉、稻草秸粉),對白腐菌在木質纖維素基質上固、液培養過程中產生的胞外羥自由基(.OH)清除能力進行了研究,并初步探析了可能的主效物質。結果表明(1)白腐菌在木質纖維素基質上不同時期的培養物均具有胞外.OH清除能力,顯示了該能力在白腐菌中的普遍性;且試驗中香菇A3培養物的胞外.OH清除能力最高,玉米秸粉比稻草秸粉更利于白腐菌培養物產生胞外.OH清除效應。(2)對香菇A3進行玉米秸粉液體培養,第8d時胞外.OH清除率達峰值42.53%,清除·OH的主效物質為胞外粗多糖,其作用占胞外液.OH總清除率效果的75.4%。
【英文摘要】 The extracellular hydroxyl radical (·OH)-scavenging ability of three white-rot fungi of different species (K1:Ganoderma lucidum, B2: Pleurots sp., A3: Lentinula edodes) on two different lignocellulose substance (cornstalk powder, straw powder) was studied, and the main scavengers were searched. The results showed that: (1)white-rot fungi have the extracellular ·OH-scavenging ability during the whole course of lignocellulose degradation, and the scavenging ability was popular in white-rot fungi. In all three strains, L.edodes A3 has the superior extracellular ·OH-scavenging ability, and the cornstalk powder was more favorable than straw powder for ·OH-scavenger production. (2) submerged culture of L.edodes A3 on cornstalk substance got the maximum scavenging efficiency of 42.53% at the 8th day, and the crude exopolysaccharide (EPS) which accounted for 75.4% of the total ·OH removal, was the main extracellular scavenger.
【中文關鍵詞】 白腐菌; 木質纖維素降解; 羥自由基清除
【英文關鍵詞】 White rot fungi; Lignocellulose degradation; Hydroxyl radical-scavenging
【文獻出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,2007年03期 【DOI】CNKI:SUN:ZSYJ.0.2007-03-019