關雪娟; 許璐; 邵彥春; 王卓仁; 陳福生; 羅信昌
湖北華中農業大學食品科技學院; 湖北華中農業大學食品科技學院; 湖北華中農業大學食品科技學院; 湖北華中農業大學植物科技學院; 湖北華中農業大學食品科技學院; 湖北華中農業大學植物科技學院 武漢430070; 武漢430070; 武漢430070; 武漢430070; 武漢430070; 武漢430070
【中文摘要】 利用簡單重復序列間擴增多態性(ISSR)對12株蘑菇屬菌株(其中菌株S1、S2為河北康保分離的菌株,具自主知識產權)和3株屬外參照菌株進行了遺傳多樣性分析,構建了遺傳相關聚類圖。本研究結果表明,河北康保分離的兩株菌株S1和S2與現存生產上應用的同類菌種存在明顯差異,同時也表明ISSR分子標記在蘑菇品種的鑒別和菌株親緣關系分析上具有較好的適用性。
【英文摘要】 12 Agaricus strains (including 2 strains which were isolated from Kangbao in Hebei province with self-owned intellectual property rights) and 3 out-genus reference strains were analyzed for the genetic diversity using inter simple sequence repeats (ISSR). The results in this paper revealed that these two isolated strains S1 and S2 had great differences with those strains in the similar species which were used in production nowadays. ISSR was a suitable molecular marker for species differentiation and genetic analysis among close related strains of Agaricus strains.
【中文關鍵詞】 蘑菇菌株; 多態性; 聚類分析
【英文關鍵詞】 Agaricus strains; Polymorphism; Cluster analysis
【基金】中國食用菌協會資助項目
【文獻出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,2007年03期 【DOI】CNKI:SUN:ZSYJ.0.2007-03-017