張瑞穎; 左雪梅; 姜瑞波
中國農業科學院農業資源與農業區劃研究所; 中國農業科學院農業資源與農業區劃研究所; 中國農業科學院農業資源與農業區劃研究所 北京100081; 北京100081; 北京100081
【中文摘要】 近年來褐斑病給我國平菇生產造成的損失日益嚴重。其癥狀主要表現為菌蓋表面出現褐色斑點;如果濕度過高,菌蓋表面能形成菌膿;如果感染原基或幼菇,會導致子實體畸形或停止發育。從病斑組織分離出病原細菌T3,根據Biolog自動鑒定系統鑒定結果、16S rDNA序列分析以及嵌套PCR檢測結果進行了綜合鑒定,確認該病原細菌為Pseudomonas tolaasii。
【英文摘要】 In resent years,significant loss of Pleurotus ostreatus which is one of most important commercially cultivated mushrooms was caused by the brown blotch disease in China.Brown blotch on pileus was formed.If humudity was higher enougy,fungal concerntration could be formed on pileus.If primordium or young fruitbody were infected,it would be malformed fruiting body or stunt.The bacteria isolated from the brown blotch was designated T3.So T3 was identified as P.talaasii on the results of Bilong system,16 SrDNA sequence analysis and nested PCR.
【中文關鍵詞】 平菇; 褐斑病; Pseudomonas tolaasii
【英文關鍵詞】 Pleurotus ostreatus; Brown blotch disease; Pseudomonas tolaasii
【基金】國家科技基礎條件平臺建設項目(2005DKA21201)
【文獻出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,2007年05期 【DOI】CNKI:SUN:ZSYJ.0.2007-05-021