郭學武; 姚麗敏; 龔建華; 肖冬光
天津科技大學天津市工業微生物重點實驗室; 湖南省衡陽市船山實驗中學; 中國科學院微生物研究所; 天津科技大學天津市工業微生物重點實驗室 天津300222; 湖南421001; 北京100080; 天津300222
【中文摘要】 采集并組織分離到假褐云斑鵝膏(Amanita pseudoporphyria)和塊鱗鵝膏(Amanita spissa)的純培養菌絲。對2株鵝膏菌的培養發現雖然分離成功率很低,但是菌絲培養相對容易,在普通瓊脂培養基上生長良好;2種鵝膏菌菌絲的生長形態和方式存在較大差別,對氨基酸的利用也存在差別,但是氣生菌絲的延伸速率卻非常相似。液體培養條件下,假褐云斑鵝膏菌絲體干重達到0.726mg·mL-1,塊鱗鵝膏干重達到0.424mg·mL-1。
【英文摘要】 Sporophores of Amanita pseudoporphyria and Amanita spissa were collected and the pure cultures of them were obtained by way of tissue cultlure. Though there is little chance to obtain pure cultures of the two Amanita species, they were relatively easy to culture and well-grown mycelia were achieved on ordinary agar culture medium. There were great variances in extension morphology and growth pattern as well as capability in utilizing amino acids, but extensional rate of aerial hypha were very similar one another. In liquid culture system, the dry mycelial weight of A. pseudoporphyria reached 0.726mg·mL-1 and A. spissa reached 0.424mg·mL-1.
【中文關鍵詞】 鵝膏菌; 外生菌根真菌; 純培養菌絲; 液體培養
【英文關鍵詞】 Amanita; Ectomycorrhiza; Liquid culture; Pure culture
【文獻出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,2008年03期 【DOI】CNKI:SUN:ZSYJ.0.2008-03-014