王磊; 邱芳萍; 王志兵; 劉洪霞; 楊尚明
長春工業大學生物工程學院
【中文摘要】 以糞鬼傘菌絲體為原料,采用超聲波輔助浸提法提取鬼傘菌多糖(CPS),并利用響應面分析法及正交實驗分析法優化提取工藝,在超聲波功率為500w、超聲波時間為20min,料液比1∶80、水浴提取溫度為80℃、提取時間為1.5h時,CPS的提取率達到最大;經氣相色譜分析,CPS由木糖、葡萄糖、半乳糖和鼠李糖4種單糖組成,摩爾比為木糖∶葡萄糖∶半乳糖∶鼠李糖=1.38∶12.85∶4.81∶1.00。
【英文摘要】 Ultrasonic-assisted technology was used to extract polysaccharide from submerged-cultured mycelium of Coprinus sterqulinus Fr. (CPS). The response surface and orthogonal design analysis were used to optimize the process conditions. At ultrasonic power 500w for 20 min,solvent-to-sample of 80∶1,water bath at 80℃ for 1.5h,the extraction rate of CPS was the biggest. CPS contained xylose,glucose,galactose and mannose at the rate of 1.38∶12.85∶4.81∶1.00,which analyzed by GC.
【中文關鍵詞】 糞鬼傘多糖; 提取工藝; 單糖組成
【英文關鍵詞】 CPS; Extraction process; Monosaccharide constitution
【基金】吉林省教育廳科技計劃項目,合同編號:200660
【文獻出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,2008年05期 【DOI】CNKI:SUN:ZSYJ.0.2008-05-019