陳自宏; 虞泓; 曾文波; 楊俊媛; 楊鐘林; 袁靜
云南大學中草藥生物資源研究所云百草實驗室
【中文摘要】 為了提高蛹草擬青霉cmily-02產蟲草素得率,采用正交實驗優化固體發酵培養基配方。以蟲草素產量為指標,通過高效液相色譜法檢測,篩選出最優固體培養基為:大米粉2.0%、青稞粉0.5%、玉米粉3.0%、黃豆粉0.5%、粗麥麩30%、KH2PO3 0.05%、MgSO4 0.1%。從培養溫度和干燥溫度2方面,探討了溫度對蟲草素產量的影響,得出產蟲草素的最佳培養溫度為28℃,最佳干燥溫度為30℃。通過固體發酵條件優化,蟲草素產量可達到1 850μg.g-1。
【英文摘要】 In order to increase the yield of cordycepin in the culture mycelia of Paecilomyces militaris cmily-02,the best solid culture medium was screened by orthogonal tests and the content of cordycepin in the culture mycelia was determined by HPLC.The results showed that the optimum medium components were rice flour 2.0%,highland barley flour 0.5%,indian meal 3.0%,soybeans meal 0.5%,outerbran 30%,KH2PO4 0.05%,MgSO4.7H2O 0.1% and so on.The optimum culture conditions were culture temperature 28℃,drying temperature 30℃ with the total yield of cordycepin reaching as high as 1 850 μg.g-1.
【中文關鍵詞】 蛹草擬青霉; 固體發酵; 正交實驗; HPLC
【英文關鍵詞】 Paecilomyces militaris; Solid fermentation; Orthogonal test; HPLC
【基金】云南省自然科學基金重點項目“云南蟲草遺傳資源及其開發利用研究”(2008CC019)
【文獻出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,2009年06期 【DOI】CNKI:SUN:ZSYJ.0.2009-06-020