楊曉彤; 張勁松; 方積年; 楊慶堯
上海師范大學菌蕈研究室; 中國科學院上海藥物研究所; 中國科學院上海藥物研究所; 上海師范大學菌蕈研究室 上海 200234 ; 上海 200032 ; 上海 200032 ; 上海 200234
【中文摘要】 用發酵培養72h的香菇菌絲體,經熱水提取,乙醇沉淀,再經二次DEAE-纖維素柱分離(Cl-型和B_4O_7)和Sephadex G-100純化,得到兩個香菇多糖——Le-2-1,Le-2-2。經HPLC(μ-Bondgel Linear柱)和醋酸纖維薄膜電泳鑒定,兩者都為均一組分,HPLC測得Le-2-1的純度為99.99%,Le-2-2為100%。氣相色譜測單糖組成、摩爾比,HPLC測分子量得Le-2-1為:Gle:Ga1:Man:Xy1:Ara:Rha=86.05:2.48:2.06:21.96:1.98:1.58,分子量:5.7×103:Le-2.2為:GlC:Gal:Man:Xyl:Ara:Rha=70.60:10.25:8.51:1.80:2.35:3.16,分子量;5.8×104。
【英文摘要】 After being extracted with hot water and precipitated with alcohol, then isolated twice by DEAE-cellulose and purified by Sephadex G-100 column chromatography, two kinds of polysaccharides--Le-2-1 and Le-2-2 were obtained from mycelia of Lentinus edodes through fermentation for 72h.As a result of the identification by means of HPLC and electrophoresis with acetate cellulose membrane, it was found that both Le-2-1 and Le-2-2 were of homogeneous compositions. The purity of Le-2-1 and Le-2-2 is 99.99% and 100% resp.. Their compositions of monosaccharides, molecular ratio and molecular weight are:Le-2-1:Gle:Gal:Man:Xyl:Ara:Rha=86.05:2.48:2.06:1.96:1.98:1.58 MW=5.7×103 Le-2-2:Gle:Gal:Man:Xyl:Ara:Rha=70.60:10.25:8.51:1.80:2.38:3.16 MW=5.8×104
【中文關鍵詞】 香菇; 菌絲體; 發酵; 多糖; 純化
【英文關鍵詞】 Lentinus edodes; Mycelia; Fermentation; Polysaccharide; Purification
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,1994年02期 【DOI】CNKI:SUN:SYJB.0.1994-02-006