潘迎捷; 鄭志仁; 李人圭
上海市農業科學院食用菌研究所; 華東師范大學生物系; 華東師范大學生物系 農業部食用菌遺傳育種重點實驗室; 上海 201106 ; 上海 200062 ; 上海 200062
【中文摘要】 本文從生化角度研究了F型(形成子實體)和N型(不形成子實體)兩類香菇菌株在生長發育過程中生化代謝活動上的差異。結果表明,在香菇的整個生長過程中,F型菌株的纖維素酶、半纖維素酶、漆酶、酸性蛋白酶和果膠酶的活性均高于N型菌株,尤其在形成子實體的第二和第三階段,纖維素酶、半纖維素酶和酸性蛋白酶的活性均呈較大幅度的增加,這種趨勢與胞外可溶性蛋白含量的增幅是一致的,同時F型菌株對培養基中木質素、纖維素和半纖維素的降解能力也高于N型菌株,說明在形成子實體的過程中,F型菌株具有比N型菌株相對更強的生化代謝能力;各種與物質降解有關酶的代謝活動在某些環節上受阻或不能有效地表達是N型菌株不能形成子實體的一個重要生理原因。
【英文摘要】 The difference in biochemistry metabolism during the growth of F type (fruiting type) and N type (non-fruiting type) L. edodes was studied. The results showed that the activities of cellulase, hemicellulase, laccase, acid protease and pectase extracted from F type were higher than that extracted from N type in general, and that the activities of cellulase, hemicellulase and acid protease increased by a big margin, which was consistent with the increment range of exocellular soluble protein content, especially at first and second flush stage. The degradation capability of F type to decompose lignocellulose was also higher than that of N type indicating that during the formation of fruitbodies, the biochemical metabolism abilities of F type were mush stronger than that of N type, and that metabolic activities of some enzymes concerned in the degradation of substance were repressed or could not be expressed effectively at some links which was an important physiological factor for non-form...更多ation of fruitbodics of N type. 還原
【中文關鍵詞】 香菇; 子實體發育; F型菌株(形成子實體); N型菌株(不形成子實體); 代謝差異
【英文關鍵詞】 Lentinula edodes; Development of fruitbody; F type (fruiting type); N type (non-fruiting type); Difference of metabolism
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,1996年03期 【DOI】CNKI:SUN:SYJB.0.1996-03-004