路等學; 李成一; 段曉紅; 王秉峰; 路曉玲; 李玉珍
甘肅省科學院生物研究所!蘭州730000; 甘肅省科學院生物研究所!蘭州730000; 甘肅省科學院生物研究所!蘭州730000; 甘肅省科學院生物研究所!蘭州730000; 甘肅省科學院生物研究所!蘭州730000; 甘肅省科學院生物研究所!蘭州730000
【中文摘要】 本研究以11個菌株為材料,對原生質體無性再生株的單、雙核比例、產菇優勢及出菇性狀進行了研究。結果表明:（1）側耳、香菇無性再生株以“鎖狀聯合”的有無可區分為無鎖狀聯合的單核株和有鎖狀聯合的雙核株,但其比例因菌株不同而迥異;（2）同一菌株的單、雙核株的菌絲生長速度、出菇期及菇產量等性狀有較大差異,雙核株多優于單核株;但原生質體雙核株與其親株雙核株相比,優劣因菌株而異;（3）無性單核株是進行細胞融合或常規雜交育種的理想材料,而原生質體雙核株可用于選出優質高產菌株。
【英文摘要】 This study dealt with the ratio between the uninuclear and binuclear strains regenerated from the protoplasts of 11 strains of edible fungi, the yield superiority and their fruiting characters. The results showed that the regenerated strains from the protoplasts of the strains of Pleurotus and Lentinula edodes could be distinguished into the uninuclear strains without "clamp connection" and the binuclear strains with "clamp connection", but the ratio between the above strains was different, that, generated from the same parent strain, the uninuclear strain was markedly different from the binuclear strain in the growth rate of mycelia, fruiting period and yield etc, the binuclear strain was mostly superior to the uninuclear strain, but whether the binuclear strain was superior to the parental binuclear strain depended on the parent strain, and that the asexual uninuclear strain was ideal material for practicing cell fusion or routine breeding, but the protoplasmatic binuclear strain cou...更多ld be used for breeding high-quality and high-yield strains. 還原
【中文關鍵詞】 側耳; 香菇; 原生質體; 無性再生株
【英文關鍵詞】 Pleurotus; Lentinula edodes; Protoplast; Asexual regenerative strain
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,1997年03期 【DOI】CNKI:SUN:SYJB.0.1997-03-000