劉國振; 賈建航; 李莉云; 劉麗娟; 劉振岳; 泰麗芳; 黃清尤
河北農業大學生物技術中心食用菌育種室; 河北農業大學生物技術中心食用菌育種室; 河北農業大學生物技術中心食用菌育種室; 河北農業大學生物技術中心食用菌育種室; 河北農業大學生物技術中心食用菌育種室; 河北農業大學生物技術中心食用菌育種室; 河北農業大學生物技術中心食用菌育種室 保定071001 ; 保定071001 ; 保定071001 ; 保定071001 ; 保定071001 ; 保定071001 ; 保定071001
【中文摘要】 本文比較了雙核菌絲制備的香菇原生質體在不同培養基上的再生率,觀察了原生質體再生過程.以存活率37%的劑量進行紫外線誘變處理后,選出102份再生克隆,從中選出在CM培養基和木屑培養基上菌絲長速較快的材料23份,進行栽培試驗,其中10份產量提高,出菇較早.在隨后的二次或三次組織分離后代的栽培試驗中,大部分材料保持了增產、早熟的性狀,經顯著性測驗.其中2份材料的產量顯著提高.本研究初步建立了用原生質體誘變技術選育香菇菌株的試驗程序.
【英文摘要】 The regeneration efficiency of Lentinula edodes protoplast prepared from dikaryon mycelium was compared on different media and the regeneration procedure was also observed under inverted microscope. 23 clones with more rapid mycelium growth rate on CM medium and sawdust medium were selected from 102 regenerated clones after being treated by UV radiation at D37 dosage. The cultivation experiment showed that 10 of the 23 regenerated clones expressed the characters of yield increment and early fruitbody formation, and that most of the progeny obtained through 2 or 3 times of tissure separation kept the characters of yield increment and early maturity and the yield of 2 regenerated clones increased significantly according to the significance test. In addition, the experiment procedure for selecting L, edodes strains with protoplast technique was also established in this study.
【中文關鍵詞】 香菇; 原生質體; 誘變; 育種
【英文關鍵詞】 Lentinula edodes; Protoplast; Induced mutation; Breeding
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,1997年04期 【DOI】CNKI:SUN:SYJB.0.1997-04-002