曾大興; 謝和
貴州大學南區園藝系; 貴州大學南區園藝系 貴陽 550025 ; 貴陽 550025
【中文摘要】 在適宜培養條件下,振蕩培養4～5d的雞縱菌(Termitomyces albuminosus)和粗柄雞■菌(T.robustus)幼嫩菌絲,經過濾洗滌收集,以0.6mol/L KCl為滲透壓穩定劑,用1％纖維素酶加0.5％蝸牛酶的混合酶液處理,于28℃酶解3～4h,可獲大量原生質體,產量達2.82×106～3.24×106/mL。但所形成的原生質體再生能力較差,再生率僅0.6％～1.2％。本文還詳細研究了菌齡、酶系統、酶解溫度和時間等多種因素對這兩種雞縱菌原生質體形成和再生的影響。
【英文摘要】 The mycelia shaking- cultured 4-5 days were gathered after being filtered and rinsed. When the mycelia were treated with a combined enzyme system composed of 1 % cellulase and 0.5% glusulase with 0. 6mol/L KC1 being used as osmotic stabilizer at 28℃ for 3-4h, high yield of protoplast (2. 82 - 3. 24×106/mL) was obtained from Termitomyces albuminosus and T. robustus. The protoplast regeneration rate of T. albuminusus and T. robustus was 0.6% - 1. 2% . While the effects of hyphal age, lytic enzyme system, osmotic stabilizer, enzy-molysis temperature and time on the preparation and regeneration of protoplast were also discussed in this paper.
【中文關鍵詞】 雞■菌; 粗柄雞■菌; 原生質體形成和再生
【英文關鍵詞】 Termitamyces albuminosus; T. robustus; Preparation and regeneration of protoplast
【基金】貴州省科學基金.項目編號:95096B—138
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,1999年02期 【DOI】CNKI:SUN:SYJB.0.1999-02-001