馮慧琴; 楊慶堯; 糜可; 沈涌
上海師范大學微生物與免疫學研究所!上海200234; 上海師范大學微生物與免疫學研究所!上海200234; 上海師范大學微生物與免疫學研究所!上海200234; 上海師范大學微生物與免疫學研究所!上海200234上海師范大學98屆學生
【中文摘要】 在液體培養條件下探討了培養基的碳源、氮源、初始 pH及其正交試驗對灰樹花菌絲生長和產糖的影響。適合的碳源是葡萄糖 +土豆 ,淀粉 ,葡萄糖 +蔗糖 ;氮源為蛋白胨 ;初始 pH為6.0。優化配方為葡萄糖 3% ,土豆汁 2 0 % ,蛋白胨 0 .6% ,豆油 0 .1% ,硫酸鎂 0 .0 5% ,磷酸二氫鉀0 .0 5% ,pH6.0。液體培養 14d產糖量最高 ,達 34 .1mg/ 10 0mL。
【英文摘要】 The effect of carbon source, nitrogen source, initial pH and orthogonal test on the mycelial growth and polysaccharide production of Grifola frondose was investigated. The results showed that the optimum carbon sources were glucose plus potato, starch and glucose plus sucrose; the optimum nitrogen source was peptone and the optimum pH was 6.0. And it was proved in our investigation that the optimal medium composition was glucose 3%, potato extract 20%, peptone 0.6%, soybean oil 0.1%, MgSO 4 0.05% and KH 2PO 4 0.05%( pH6.0),and that the highest yield of polysaccharide was 34.1mg/100mL after fourteen days of liquid culture.
【中文關鍵詞】 灰樹花; 菌絲體; 液體培養; 多糖
【英文關鍵詞】 Grifola frondosa; Mycelium; Liquid culture; Polysaccharid
【基金】上海市教委發展基金資助項目! (97D14)
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2000年02期 【DOI】CNKI:SUN:SYJB.0.2000-02-001