彭衛紅; 肖在勤; 鄭林用; 甘炳成
四川省農業科學院土壤肥料研究所; 四川省農業科學院土壤肥料研究所; 四川省農業科學院土壤肥料研究所; 四川省農業科學院土壤肥料研究所 成都610066 ; 成都610066 ; 成都610066 ; 成都610066
【中文摘要】 以長根菇和雞【為親本進行屬間不對稱融合研究 ,從原生質體再生菌株中得到能夠雙核化的單核無鎖狀聯合的菌株 2 0株。栽培結果顯示 ,這類菌株不能形成子實體 ,且菌株間菌絲的生長差異顯著。但在特定的培養條件下 ,單核菌株均可雙核化形成雙核菌株 ,雙核菌株具有出菇能力 ,菌株間子實體顏色、菌柄著生方式及菌絲生長速度等與親本有明顯的差異
【英文摘要】 Through asymmetric fusion between O.radicata and T.albuminosus under high pH and high Ca 2+ conditions, 20 monokaryotic strains without clamp connection which could be dicaryotized were obtained from regenerative protoplast strains. The cultural test indicated that no fruitbody was developed from monokaryotic strains and the mycelium growth characteristics between monokaryotic strains were significantly different. Under given conditions, the monokaryotic strains were dicaryotized into dicaryotic strains which could form fruitbody. But the fruitbody colour, the stipe insertion mode and the mycelium growth rate of dicaryotized strains were significantly different from their parent strains and also significantly different between the dicaryotic strains tested.
【中文關鍵詞】 長根菇; 雞【; 不對稱融合; 生長特性; 雙核化
【英文關鍵詞】 Oudemansiella radicata; Termitomyces albuminosus; Asymmetric fusion; Growth characteristic; Dicaryotization
【基金】農業部重點資助項目 (99-農 - 0 6 )
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2002年01期 【DOI】CNKI:SUN:SYJB.0.2002-01-000