李三暑; 林新堅; 陳惠成; 雷錦桂; 林勇; 江枝和
福建省農科院土壤肥料研究所; 福建省農科院土壤肥料研究所; 福建省農科院土壤肥料研究所; 福建省農科院土壤肥料研究所; 福建省農科院土壤肥料研究所; 福建省農科院土壤肥料研究所 福州350013 ; 福州350013 ; 福州350013 ; 福州350013 ; 福州350013 ; 福州350013
【中文摘要】 以 4個姬松茸菌株和 2個雙孢蘑菇菌株為材料 ,用 2 0個隨機引物對它們進行RAPD擴增。通過對供試菌株遺傳相似系數的估算和系統聚類分析 ,構建姬松茸和雙孢蘑菇遺傳相關樹狀聚類圖譜。結果表明 ,進行RAPD擴增的 2 0個隨機引物中 ,有 12個能產生三種帶型。當遺傳相似系數升至 0 .8713時 ,這 6個菌株被分為 3類 ,第一類為菌株 18和 2 6 ,第二類為菌株 13和 12 ,第三類為菌株 33和 4 5。
【英文摘要】 Four strains of Agaricus blazei and two strains of Agaricus bisporus were amplified by RAPD (Random Amplified Polymorphic DNA) technique with 20 random primers. A dendrogram was generated through the assessment of the similarity coefficient of these six strains tested and systematic clustering analysis. The result showed that among 20 primers tested, 12 primers could produce three band types of DNA fragments. When the similarity coefficient raised to 0.8713, six strains tested were divided into three classes. The first class included strains 18 and 26, the second class included strains 12 and 13 and the third class included strains 33 and 45.
【中文關鍵詞】 姬松茸; 雙孢蘑菇; RAPD
【英文關鍵詞】 Agaricus blazei; Agaricus bisporus; RAPD(Random Amplified Polymorphic DNA)
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2002年03期 【DOI】CNKI:SUN:SYJB.0.2002-03-000