【作者】潘慧; 徐章逸; 邊銀丙
【Author】PAN Hui1; XU Zhangyi1; BIAN Yinbing1; 21Institute of Applied Mycology; Huazhong Agricultural University; Wuhan; Hubei 430070; China; 2 Research Center of Edible Mushroom Engineering and Technology of Hubei; Wuhan; Hubei 430070; China
【作者機構】華中農業大學應用真菌研究所; 湖北省食用菌工程技術研究中心
【摘要】 以杏鮑菇(Pleurotus eryngii)6號和ACCC50894為親本,分別采用單孢雜交和原生質體單核體雜交方式獲得65和37個雜交子,觀測雜交子及親本菌絲體生長情況,選擇長勢好及生長快速的雜交子進行栽培出菇試驗。采用灰色關聯度分析法對15個生長勢較強的雜交子從7個性狀方面進行綜合評價,結果表明,單孢雜交獲得的雜交子D12、D18、D20、D30、D19、D31、D17和D48關聯度排名先于兩親本,農藝性狀和子實體性狀表現較好;原生質體單核體雜交菌株Y29和Y30關聯度排名介于兩親本之間。
【Abstract】 Mating experiments involving either monokaryotic basidiospores or protoplast monokaryons derived from Pleurotus eryngii parental strains No.6 and ACCC50894 were used to generate 65 and 37 hybrid strains,respectively.Fruiting tests were carried out on hybrids selected on the basis of mycelial growth rate and growth vigor,and fruit bodies were successfully obtained from 15 hybrids.Grey correlation analysis based on a comprehensive evaluation of seven selected agronomic traits revealed that hybrids D12,D18,D20,D30,D19,D31,D17 and D48 obtained from monospore crossings were superior to the two parental strains,while hybrids Y29 and Y30 obtained from protoplast monokaryon crossings ranked between the two parental strains.
【關鍵詞】 杏鮑菇; 單孢雜交; 原生質體單核體雜交; 農藝性狀; 灰色關聯度分析
【Key words】 Pleurotus eryngii; monospore crossbreeding; protoplast monokaryon crossbreeding; agronomic character; grey correlation analysis
【基金】公益性行業科技(農業)專項(編號:NYHYZX07-008);; 武漢市科技產業化專項(編號:20062001011)的部分研究內容
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2010年02期
【DOI】CNKI:SUN:SYJB.0.2010-02-005
【分類號】S646.1