【作者】王欽博; 楊焱; 劉艷芳; 高君輝; 張勁松; 唐慶九; 馮娜; 周帥
【Author】WANG Qinbo1; 2; YANG Yan1; LIU Yanfang1; GAO Junhui1; ZHANG Jinsong1; TANG Qingjiu1; FENG Na1; ZHOU Shuai11National Engineering Research Center for Edible Fungi Biotechnology and Engineering; Key Laboratory of Applied Mycological Resources and Utilization; Ministry of Agriculture; Key Laboratoryof Agricultural Genetics and Breeding of Shanghai; Research Center of Natural Medicinal Resources; Instituteof Edible Fungi; Shanghai Academy of Agricultural Sciences; Shanghai 201106; China; 2College of Life and Environmental Sciences; Shanghai Normal University; Shanghai 200234
【作者機構】國家食用菌工程技術研究中心農業部應用真菌資源與利用重點開放實驗室上海市農業遺傳育種重點開放實驗室上海市農業科學院天然藥物研究與開發中心上海市農業科學院食用菌研究所; 上海師范大學生命與環境科學學院
【摘要】 測定了一年生、兩年生桑黃(Phellinus baumii)子實體水提物中的粗多糖、醇提物中的黃酮和三萜含量,并研究了不同生長年份子實體水提粗多糖和醇提物體外刺激淋巴細胞生長、抑制L1210腫瘤細胞生長的作用及抗氧化活性。結果表明,一年生桑黃子實體醇提物中黃酮、三萜和水提物中粗多糖的含量略高于兩年生桑黃;當水提粗多糖濃度為10μg/mL時,SH2-W對體外淋巴細胞的增殖率高于SH1-W,當濃度為50和200μg/mL時,兩組樣品間無顯著差異;兩種桑黃醇提物對體外腫瘤細胞L1210的抑制作用隨濃度增大而增強,相同劑量下,兩組樣品間無差異;相同濃度下SH1-E對H2O2的清除率高于SH2-E,但兩組樣品DPPH.的清除率和還原力無顯著差異。
【Abstract】 One-and two-year-old Phellinus baumii fruit bodies were extracted with water(SH1-W and SH2-W,respectively) or ethanol(SH1-E and SH2-E,respectively),and the polysaccharide content of the aqueous extracts,and the flavone and triterpene content of the ethanolic extracts,were determined.Polysaccharide levels in fraction SH1-W,and flavone and triterpene levels in fraction SH1-E,were higher than in the corresponding extracts of two-year-old sporophores.At 10 μg/mL concentration,fraction SH2-W was more effective than fraction SH1-W at stimulating lymphocyte proliferation,but there was no significant difference in the stimulating effects of the two extracts at higher concentrations(50 and 200 μg/mL).Inhibition of L1210 tumor cell growth in vitro by fractions SH1-E and SH2-E increased with increasing extract concentrations but there was little difference in the inhibitory effects of the two extracts at the same concentration level.The H2O2 scavenging capacity of SH1-E was higher compared with S...更多H2-E at the same concentration,but the two extracts exhibited similar DPPH· scavenging activity and reducing power. 還原
【關鍵詞】 桑黃子實體; 粗多糖; 黃酮; 抗氧化; 淋巴細胞增殖
【Key words】 Phellinus baumii; fruit body; polysaccharide; flavonoid; antioxidant activity; lymphocyte proliferation
【基金】桑黃黃酮生物制品制備技術中試優化(編號:093919N0900)的部分研究內容
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2010年02期
【DOI】CNKI:SUN:SYJB.0.2010-02-015
【分類號】S567.39