程東升; 鄒莉; 潘學仁
東北林業大學
【中文摘要】 探討了外生菌根菌耳環乳牛肝（Ｓｕｉｌｌｕｓｇｒｅｖｉｌｌｅｉ）的原生質體制備及再生條件。用ＭＹＧ培養基培養菌絲７天、宋用１．５％Ｌｙｗａｌｌｚｙｍｅ，以０．６ｍｏｌ甘露醇為穩滲劑，在酶解液ｐＮ值５．５，溫度３１℃，酶解４ｈｒ的條件下，每０．１ｇ濕菌絲的原生質體產量這６×１０￣６／ｍｌ。用ＳＯ培養基培養菌絲能提高原生質體產量，但再生率下降。綜合產量和再生率考慮以ＭＹＧ培養菌絲效果較好。
【英文摘要】 Conditions for preparingthe protoplasts from mycelia of ectomycorrhizal fungus SMillus grevillei were examined.The highest production of protoplasts(6 ×10￣6/ml per 0.1g fresh mycelia) was abtained with the enzyme mixture of pH 5.5 containing 1.5% Lywallzyme,0.6mnol/L mannitol as osmotic stabilizer,and incubated at 31℃, from 7-day age mycelia cultured in MYG medium. UsingSO miedium instead of MYG doubled the protoplast productlon, but markedly decreased the plating efficiency. It is concl-uded that MYG is a better medium than SO for the preparation of protoplasts from Suillus previlllei.
【中文關鍵詞】 外生菌根菌; 原生質體制備; 細胞工程
【英文關鍵詞】 Ectomycorrhizal fungi; Protopiast prepartion; Cytological engi-neering
【基金】國家教委資助基金
【文獻出處】 中國食用菌,EDIBLE FUNGI OF CHINA,編輯部郵箱,1994年02期 【DOI】CNKI:SUN:ZSYJ.0.1994-02-009