程南升; 潘學仁
東北林業大學林學系
【中文摘要】 比較了美味側耳不同菌體組織中５種酶的同工酶譜變化。酯酶（Ｅ５Ｔ）和蘋果酸脫氫酶（ＭＤＨ）在子實體中的酶帶數和活性均高于液培菌絲。過氧化物酶（ＰＯＤ）的情況則正好相反。乙醇脫氫酶（ＡＤＨ）和山梨醇脫氫酶（ＳＯＤＨ）的酶帶只在子實體中發現。酶譜隨子實體部位而變異的進度同酶種類有關，ＰＯＤ變化較大，ＥＳＴ和ＭＤＨ較不明顯，ＡＤＨ和ＳＯＤＨ沒有變化。本實驗結果為今后以這５種酶為生化遺傳標記研究美味側耳提供了基礎依據。
【英文摘要】 Variation of isozyme patterns for five enzymes in different tissues of edible fungus PIeurotus sapidus was investigated,More isozyme bands and higher activity for esterase(EST)and malate dehydrogenase(MDH)were found in fruit body than in liquid-cultured mycelia,and the situation for perozidase(POD),however,was just on the con-trary.Activity of alcohol dehydrogenase(ADH) and sorbitol dehydrogenase(SODH) wasdetected only frops fruit body.The tissue specificity of isozyme patterns in fruit bodyvaried alnong the enzymes,most notable for POD,less for EST and MDH,and none forADH and SODH. The present test provided basis for further studies on Pleurotus sapidusconcerning the usage of these enzymes as biochemical or genetical markers.
【中文關鍵詞】 美味側耳; 同工酶; 組織特異性
【英文關鍵詞】 Pleurotus sapidus; Isozyme; Tissue specificity
【文獻出處】 中國食用菌,EDIBLE FUNGI OF CHINA,編輯部郵箱,1995年06期 【DOI】CNKI:SUN:ZSYJ.0.1995-06-014