范雷法; 潘慧娟
浙江農科院園藝研究所; 浙江農科院園藝研究所 浙江杭州310021 ; 浙江杭州310021
【中文摘要】 本實驗首先確定潮霉素濃度對香菇和平菇菌絲和原生質體再生的影響。其次進行了溫度和時間對平菇原生質體滅活效果的研究。然后利用香菇和平菇再生原生質體對潮霉素抗潮霉素的差異,結合平菇原生質體滅活為融合子篩選依據,在融合劑誘導下獲得4株融合子。
【英文摘要】 Hygromycin B resistance was studied on the mycelium and protoplast of Lentinula edodes and Pleurotus ostreatus.Test was done on inactivation of protoplast of P.ostreatus by temperature and duration.The protoplasts were prepared and fused with fusogen(PEG)after inactivation of protoplasts of Pleurotus ostreatus at 60℃ for 2h.Four fusants were obtained in the selection medium containning hygromycin B.
【中文關鍵詞】 香菇; 平菇; 原生質體融合; 融合子; 潮霉素
【英文關鍵詞】 Lentinula edodes; Pleurotus ostreatus; Protoplast fusion; Fusant; Hygromycin B
【文獻出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,2005年05期 【DOI】CNKI:SUN:ZSYJ.0.2005-05-006