胡文彬; 馬海樂; 周存山
江蘇大學生物與環境工程學院; 江蘇大學生物與環境工程學院; 江蘇大學生物與環境工程學院 鎮江212013; 鎮江212013; 江蘇省農產品生物加工與分離工程技術研究中心; 鎮江212013; 鎮江212013
【中文摘要】 研究了桑黃菌在發酵過程中菌絲體及胞內外多糖產量變化趨勢,碳源、氮源以及接種量、搖床轉速、溫度和pH值等因素對桑黃菌液體深層發酵菌絲產量的影響。結果表明:玉米粉為最佳碳源,豆餅粉為最佳氮源,優化培養基配方為:玉米粉4.0%、豆餅0.5%、KH2PO40.1%、MgSO4.7H2O0.05%;最適菌絲體生長的液體發酵條件:培養溫度26℃,搖瓶轉速130rpm,pH值6.5,接種量15%￣20%,培養時間7d。
【英文摘要】 The change tendency of output from intracellular and extracellular polysaccharide in the fermentative period for the mycelium of Phellinus igniarius was studied.Carbon source,nitrogen source,inoculating amount,rotational speed,temperature and pH etc.affected the output of mycelium in liquid submerged fermentation for Phellinus igniarius.The results showed that maize meal was the optimum carbon source,and bean cake was the best nitrogen source.The fine formula of medium was: maize meal 4.0%,bean cake 0.5%,KH2PO40.1%,MgSO4·7H2O0.05%.The most suitable fermentative condition was: cultivated temperature 26℃,rotational speed130rpm,pH6.5,inoculating amount 15%20%,growth time 7d.
【中文關鍵詞】 桑黃; 深層發酵; 菌絲產量; 多糖
【英文關鍵詞】 Phellinus igniarius; Submerged fermentation; Mycelium yield; Polysaccharide
【文獻出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,2006年03期 【DOI】CNKI:SUN:ZSYJ.0.2006-03-017