柯麗霞; 楊曉彤
安徽師范大學生命科學學院; 上海師范大學微生物學與免疫學研究所 安徽蕪湖241000; 上海200234
【中文摘要】 研究了松乳菇深層培養所需的碳、氮源及其交互作用。在供試的多種碳、氮源中,最適合菌絲體生長和胞外生物聚合物產生的碳源是蔗糖和葡萄糖;最適合菌絲體生長的氮源是牛肉膏和蛋白胨,最適合胞外生物聚合物產生的氮源是黃豆粉,松乳菇不能利用尿素和亞硝酸鹽。碳、氮源交互試驗結果表明:松乳菇菌絲體生長和胞外生物聚合物產生的優良培養基為培養基1:蔗糖1.5%、可溶性淀粉1.5%、牛肉膏0.25%、黃豆粉1%、硝酸鈉0.1%;菌絲體和胞外生物聚合物的生物量分別為0.923g/100mL和80.1mg/100mL;培養基2:蔗糖1.5%、可溶性淀粉2%、牛肉膏0.1%、黃豆粉0.75%、硝酸鈉0.3%;菌絲體和胞外生物聚合物的生物量分別為0.927g/100mL和83.2mg/100mL。
【英文摘要】 This paper presents a research on carbon and nitrogen resources by submerged culture of Lactarius deliciosus.The result show that the sucrose and the glucose are the optimal carbon resources for mycelial growth and exo-biopolymer production;the optimal nitrogen resources for mycelial growth are the beef extract and pepton,the optimal nutrogen resources for exo-biopolymer production are soy powder and beef extract,urea and nitrite can not be utilized.The orthogonal test results show that the optimal medium for mycelial growth and exo-biopolymer production are media 1: sucrose 1.5%,soluble starch 1.5%,beef extract 0.25%,soy powder 1%,NaNO_3 0.1%,the mycelial and exo-biopolymer output in this media are 0.932g/100mL and 80.1mg/100mL seperatively;and media 2: sucrose 1.5%,soluble starch 2%,beef extract 0.1%,soy powder 0.75%,NaNO_3 0.3%,the mycelial and exo-biopolymer output in this media are 0.927g/100mL and 83.2mg/100mL seperatively.
【中文關鍵詞】 松乳菇; 深層培養; 菌絲體; 胞外生物聚合
【英文關鍵詞】 Lactarius deliciosus; Submerged culture; Mycelia; Exo-biopolymer; Carbon and nitrogen resources
【基金】安徽省教育廳自然科學基金資助項目(2004kj176)
【文獻出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,2007年01期 【DOI】CNKI:SUN:ZSYJ.0.2007-01-014