李成一; 路等學; 盧洪英; 王秉峰
甘肅省科學院生物研究所; 甘肅省科學院生物研究所; 甘肅省科學院生物研究所; 甘肅省科學院生物研究所 蘭州 730000 ; 蘭州 730000 ; 蘭州 730000 ; 蘭州 730000
【中文摘要】 側耳、香菇皆屬四極性異宗結合食用菌,其異核雙核菌絲體均具“鎖狀聯合”形態標記。本研究以PEG-Ca++為誘導劑,以紫孢側耳和香菇Cr-20的原生質體再生單核體為親株,進行側耳與香菇的屬間原生質體融合,結果從1022個融合再生體中鏡檢出16個具鎖狀聯合遺傳標記的融合株。對1～4號融合株與親株進行的拮抗反應、雙單交配、過氧化物同功酶譜分析及菇形特征對比皆證明融合株確具雙親雜合體特征。出菇驗證表明,融合株菌絲體長速均快于親株,出菇期早,菇產量近于或高于親株側耳的水平。
【英文摘要】 Pleurotus sapidus and Lentinus edodes are tetrapolarity heterothallic edible fungus, the heterokaryotic dikaryotic mycelia had morphology marker of clamp conncnction (C. C. ). In this study, intergeneric protoplast fusion was carried out using regenerative uninuclear protoplasts from P. sapidus and L. edodes Cr-20 as parent strains, using PEG-Ca++ as inducer. 16 fusion strains with genetic marker of C. C. were obtained from 1022 regenerative strains. Antagonistic experiment, di-mon mating, peroxidase isoenzyme pattern anylysis, comparison of fruit body morphology and characters were conducted for 4 fusion strains. The test showed that the fusion strains have hybrid characters of the parents. In addition, the yeild of the fusion strains, with mycelia growth rate faster and fruiting period earlier than that of their parents, was comparable to or higher than that of Pleurotus parent.
【中文關鍵詞】 側耳; 香菇; 原生質體融合育種; 具鎖狀聯合遺傳標記; 出菇驗證
【英文關鍵詞】 Pleurotus sapidus; Lentinus edodes; Protoplast fusion breeding; Genetic marker with C. C.; Fruiting test and verification
【基金】甘肅省科學院1989年計劃項目
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,1994年01期 【DOI】CNKI:SUN:SYJB.0.1994-01-003