何強泰; 陸佩洪; 余多慰
南京師范大學生物系細胞遺傳教研室!南京210024; 南京師范大學生物系細胞遺傳教研室!南京210024; 南京師范大學生物系細胞遺傳教研室!南京210024
【中文摘要】 糙皮側耳菌絲體在含2.5％溶壁酶的0.6M KC1高滲穩定液中,25℃說解48～57小時,或在37℃酶解4小時后再在25℃酶解47小時,皆可產生直徑36.84μm左右的巨原生質體,較一般正常原生質體增大7～10倍左右。觀察巨原生質體超微結構,發現其中液泡體積相應增加、線粒體和內容物增多、細胞核數目沒有變化,并觀察到巨原生質體的再生。
【英文摘要】 When mycelia of Pleurotus ostreatus were incubated in 0.6M KC1 hypertonic solution with 2.5% lywallzyme at 25℃ for 48-57 hr.; or after enzymolysis at 37℃ for 4 hr. and incubation at 25℃ for 47 hr., the giant protoplast with diameter of 36.84um was obtained. Its average diameter could be 7-10 times greater than that of normal protoplast. Ultrastructure of the giant protoplast was studied with electron microscope. The results showed that the vacuole volume became large, the contents and the mitochondrion increased, but the numbers of nuclei were unchanged. And the regeneration of giant protoplast was also observed.
【中文關鍵詞】 食用菌; 巨原生質體; 超微結構
【英文關鍵詞】 Edible fungi; Giant protoplast; Ultrastructure
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,1996年02期 【DOI】CNKI:SUN:SYJB.0.1996-02-007