潘迎捷; 鄭志仁; 李人圭
上海市農業科學院食用菌研究所農業部食用菌遺傳育種重點實驗室!上海201106; 華東師范大學生物系!上海200062; 華東師范大學生物系!上海200062
【中文摘要】 本文測定了溫度對香菇胞外纖維素酶和半纖維素酶活性的影響。木屑基質培養90天的香菇胞外游紙纖維素酶（C_1酶）、羧甲基纖維素酶（C_X酶）和半纖維素酶（H_C酶）的最適作用溫度分別是55℃、55～60℃和50℃。在50～55℃條件下保溫1小時后,C_1酶和H_C酶活性大幅度下降,而C_x酶活性下降較為緩慢,具有較高的熱穩定性。本文提出相對活力系數（Relative activity coefficient,RAC）作為表示香菇菌絲生長溫度與酶最適作用溫度之間關系的一個參數。
【英文摘要】 The effect of temperature on extracellular ctllulasc and hemicellulase was detected. After 90 days cultivation on sawdust medium, it was found that the best suitable temperature of extracellular cellulaso, extracellular carboxymcthyl cellulase and extracellular hemicellulase was 55℃), 55-60℃ and 50℃ respectively. After 1h treatment at 50-55℃, cellulase and hemicellulase activity dropped significantly, but carboxymethyl cellulase activity decreased slowly. The results indicated that carboxymethyl cellulase was more thermostable. It is considered that the relative activity coefficient could be used as index to show the relationship between Lentinula edodes mycelium growth temperature and the best suitable temperature of enzyjne activity.
【中文關鍵詞】 香菇; 菌絲生長溫度; 纖維素酶; 半纖維素酶; 相對活力系數
【英文關鍵詞】 Lentinula edodes: Mycelium growth temperature; Cellulase; Hemicellulase; Relative activity coefficient
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,1996年02期 【DOI】CNKI:SUN:SYJB.0.1996-02-000