譚琦; C.Peter Romaine; Beth Schlagnhaufer
上海市農業科學院食用菌研究所; Penn.State University; Penn.State University 農業部食用菌遺傳育種重點開放實驗室 上海農業遺傳育種重點實驗室; 上海 201106 ; U.S.A ; U.S.A
【中文摘要】 本文選用了102個分離于雙孢蘑菇菇床上的木霉菌株(Trichoderma sp.),其中93株來自美國,3株來自加拿大,6株來自英國。利用RAPD技術進行遺傳差異測定。25個隨機引物共擴增出223條DNA帶,在此基礎上分析并構建了遺傳相關聚類圖。結果表明,美國與加拿大雙孢蘑菇菇床上分離的木霉菌株相似性極高,達95％左右;而與英國的木霉菌株遺傳距離較遠,遺傳相似性低于50％。
【英文摘要】 The genetic diversity analysis of 102 Trichoderma sp. strains isolated from Agaricus bisporus cultural bed, of which 93 were from America, 3 were from Canada and 6 were from England, was conducted by RAPD in this study. A total of 223 products were amplified with 25 arbitrary primers and genetic similarity dendrogram was constructed on it. It was noted that less genetic diversity existed between American and Canadian strains of Trichoderma with a similarity index of about 95 % . But more genetic diversity existed between English and American strains of Trichoderma and the genetic similarity index was below 50 % .
【中文關鍵詞】 木霉; 雙孢蘑菇; RAPD
【英文關鍵詞】 Trichoderma sp. ; Agaricus bisporus; RAPD
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,1998年02期 【DOI】CNKI:SUN:SYJB.0.1998-02-006