馬愛民; 潘迎捷; 關海山
華中農業大學應用真菌研究所; 上海市農業科學院食用菌研究所; 香港中文大學生物系 武漢 430070 ; 上海 201106 ; 香港
【中文摘要】 本研究以雙孢蘑菇原生質體同核體及雜交異核體為材料,用5對隨機雙引物進行RAPD分析。結果表明,RAPD反應擴增出的特異DNA譜帶能夠將原生質體同核體與異核體出發菌株以及雜交異核體與同核體親本明顯區別開來。因此,RAPD分析是一種快速有效的手段,能夠在雙孢蘑菇的遺傳研究和菌種改良中發揮重要作用。
【英文摘要】 Random Amplified Polymorphic DNA( RAPD) analysis was conducted with 5 random paired primers in protoplasted homokaryons and hybrid heterokaryons of Agaricus bisporus in this study. The results revealed that protoplasted homokaryons and hybrid heterokaryons were distinguished obviously from relevant heterokaryons and parental homokaryons by some amplified polymorphic DNA bands. It was suggested that RAPD analysis was a useful technique, and that it could play an important role in genetic research and strain improvement of Agaricus bisporus .
【中文關鍵詞】 雙孢蘑菇; 原生質體同核體; 雜交異核體; RAPD
【英文關鍵詞】 Agaricus bisporus; Protoplasted homokaryon; Hybrid heterokaryon; RAPD
【基金】上海市科委資助項目
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,1998年03期 【DOI】CNKI:SUN:SYJB.0.1998-03-000