林芳燦; 汪中文; 代江紅
華中農業大學應用真菌研究所!武漢430070; 華中農業大學應用真菌研究所!武漢430070; 華中農業大學應用真菌研究所!武漢430070
【中文摘要】 在香菇中 ,由于沒有明確的形態差別 ,3種不親和交配反應 ,即A =B =,A =B≠andA≠B =無法彼此區別。本研究將一種稱為OWE -SOJ的新技術應用于香菇單孢分離物的對峙培養。結果表明 ,A≠B≠形成具有鎖狀聯合的絨毛狀菌落 ,A≠B =形成沒有鎖狀聯合的柵欄型菌落。A =B =和A =B≠都形成無鎖狀聯合的絨毛狀菌落 ,但通過一個附加的常規交配試驗 ,二者易于相互區別。因此 ,在通常的交配試驗基礎上 ,應用OWE -SOJ技術準確鑒定 4種不同的交配反應是可行的。
【英文摘要】 Three incompatible mating interactions, A=B=, A=B≠ and A≠B=, are hardly distinguishable from each other for the absence of definite morphological differences in Lentinula edodes. In this study, a new technique known as OWE-SOJ was applied to the confrontation culture of monosporous isolates of L.edodes. The result indicated that A≠B≠ formed a fluffy colony with clamp connection, while A≠B= formed a barrage colony without clamp connection. Both A=B= and A=B≠ formed fluffy colonies without clamp connection, but they could be distinguished easily from each other by an additional routine mating test. Therefore, it is feasible to apply OWE-SOJ technique to determine four different mating interactions accurately on the basis of an ordinary mating test.
【中文關鍵詞】 香菇; OWE-SOJ技術; 交配反應
【英文關鍵詞】 Lentinula edodes ; OWE-SOJ technique; Mating interaction
【基金】SupportedbyNationalScienceFoundationofChina! (39770 0 15 )
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2000年04期 【DOI】CNKI:SUN:SYJB.0.2000-04-002