朱朝輝; 陳明杰; 譚琦; 賀冬梅; 潘迎捷
南京農業大學資環學院微生物系!南京210000; 農業部食用菌遺傳育種重點開放實驗室!上海市農業遺傳育種重點實驗室; 上海市農業科學院食用菌研究所; 上海201106; 農業部食用菌遺傳育種重點開放實驗室!上海市農業遺傳育種重點實驗室; 上海市農業科學院食用菌研究所; 上海201106; 農業部
【中文摘要】 在 8個香菇菌株原生質體單核體交配型的測定中 ,有 4個菌株測定出兩種交配型 ,其余 4個菌株只測得一種交配型。在得到兩種交配型的菌株中 ,其交配型比例也不是 1:1。進一步的實驗表明 ,產生這一現象的主要原因與兩種交配型原生質體單核體的再生能力不同有關。在申香 2號菌株 10 7個單核體中 ,AxBx交配型只有 2 6個 ,而且都是在再生階段的前期產生的 ,后期出現的單核體則都是AyBy型菌落。
【英文摘要】 From the result of determining protoplasted monokaryon mating types in 8 Lentinula edodes cultivated strains, it was discovered that two mating types were only found in 4 strains, and only one mating type was found in the rest of other 4 strains, and that in those 4 strains with two mating types, the ratio of two kinds of mating type was not 1∶1. Further experiments suggested that protoplasted monokaryons with different kinds of mating type had different ability of regeneration. In 107 monokaryons of Shenxiang 2 (SX-2), mating type of AxBx was only found in the early regeneration period of 26 protolasted monokaryons, while mating type of AyBy was only found during the later period of protoplasted monokaryon regeneration.
【中文關鍵詞】 香菇; 原生質體單核體; 交配型
【英文關鍵詞】 Lentinula edodes; Protoplasted monokaryon; Mating type
【基金】國家自然科學基金資助項目!編號 :39870 5 40
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2000年04期 【DOI】CNKI:SUN:SYJB.0.2000-04-000