譚琦; 楊建明; 陳明杰; 賀冬梅; 潘迎捷; 黃為一
南京農業大學資源與環境科學學院!南京210095; 安徽技術師范學院!鳳陽233100; 上海市農業科學院食用菌研究所!農業部食用菌遺傳育種重點開放實驗室; 上海市農業遺傳育種重點實驗室; 上海201106; 上海市農業科學院食用菌研究所!農業部食用菌遺傳育種重點開放實驗室; 上海市農業遺傳育
【中文摘要】 利用RAPD技術 ,對香菇非對稱雜交的親本與后代之間的遺傳相關性進行了分析。2 0個隨機引物共擴增出 4 7條DNA片段 ,在此基礎上分析構建了遺傳相關聚類圖。結果表明 ,非對稱雜交的后代 ,其遺傳距離與單核受體較近 ,遺傳相似性在 85%左右 ,而與雙核供體遺傳距離較遠 ,遺傳相似性在 61%左右
【英文摘要】 The genetic similarity among the acceptor parent, donor parent and filial generations of nonsym- metrical crossing Lentinula edodes was analyzed with RAPD technigue. 47 DNA fragments were amplified by 20 random primers. And the dendrogram of genetic correlation was constructed on this basis. The result showed that the genetic characters of nonsymmetrical crossing filial generations were similar to that of the mononuclear accepter-parent with a genetic similarity index of about 85%, and that more genetic diversity exsisted between the nonsymmetrical crossing filial generation and the binuclear donor parent with a genetic similarity index of about 61%.
【中文關鍵詞】 香菇; 非對稱雜交; RAPD; 遺傳分析
【英文關鍵詞】 Lentinula edodes; Nonsymmetrical crossing; RAPD; Genetic analysis
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2001年02期 【DOI】CNKI:SUN:SYJB.0.2001-02-001