劉艷芳; 楊焱; 賈薇; 張勁松; 唐慶九
上海市農業科學院食用菌研究所; 上海市農業科學院食用菌研究所; 上海市農業科學院食用菌研究所; 上海市農業科學院食用菌研究所; 上海市農業科學院食用菌研究所 上海201106 ; 上海201106 ; 上海201106 ; 上海201106 ; 上海201106
【中文摘要】 采用不同的碳氮源及不同比例的混合碳氮源對猴頭菌進行深層發酵培養,以菌絲體生物量為主要指標篩選出菌絲體生物量較高的培養基優化組合。同時,初步研究了搖瓶繼代次數對菌絲體生物量的影響。結果表明,最優培養基為葡萄糖3%,可溶性淀粉2%,酵母膏1%,麥麩1%,KH2PO40.1%,MgSO4·7H2O0.05%;最佳搖瓶繼代次數為2次。
【英文摘要】 The screening of the optimum submergedculture medium for Hericium erinaceus was carried out with the mycelia biomass being taken as the major index in the submergedculture of H. erinaceus mycelia,using different single Csources,Nsources and combined Csources and Nsources mixed with different ratios to select the optimized combined medium with higher mycelial biomass. And the effect of the subculture times on the submerged culture of H. erinaceus mycelia was also studied. The results showed that the compositions of the optimum submergedculture medium were as follows: glucose 3%,soluble starch 2%,yeast extract 1%,wheat bran 1%, KH2PO4 0.1%,MgSO4·7H2O 0.05%, and the optimum subculture time was two.
【中文關鍵詞】 猴頭菌; 深層發酵; 培養基篩選
【英文關鍵詞】 Hericium erinaceus; Submerged-culture; Screening of the culture medium
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2003年03期 【DOI】CNKI:SUN:SYJB.0.2003-03-006