江艷華; 江曉路; 王鵬; 王首燕
中國海洋大學生命科學與技術學部食品工程系; 中國海洋大學生命科學與技術學部食品工程系; 中國海洋大學生命科學與技術學部食品工程系; 中國海洋大學生命科學與技術學部食品工程系 青島 266003 ; 青島 266003 ; 青島 266003 ; 青島 266003
【中文摘要】 研究了白靈菇液體培養條件,確定最佳培養基配方為10%(w/v)麩皮,1%蔗糖,0.3%蛋白胨,0.1%KH2PO4,0.05%MgSO4;最佳培養條件為pH7.0,溫度25℃,接種量10%(v/v),培養基裝量100mL/250mL,培養時間7d。栽培試驗表明,液體菌種制種時間比固體菌種的少13～18d,頭潮菇出菇時間縮短了10～20d。
【英文摘要】 The optimal fermentation medium and the optimal culture conditions for submerge-cultured Pleurotus nebrodensis were studied in this paper. The results showed that the optimal fermentation medium was composed of wheat bran 10 % (w/v) , sucrose 1 % , peptone 0.3%, KH2 PO4 0. 1 % , MgSO4 0. 05 % ; the optimal culture conditions were pH 7. 0, inoculation rate 10% (v/v) , medium capacity 100mL/250mL, culture time 7d. The results of comparative cultivation of Pleurotus nebrodensis showed that the preparing time of submerged spawn was 13-18d shorter than that of the solid spawn and the fruitbody formation time of the first flash was 10-20d earlier than that of the solid spawn.
【中文關鍵詞】 白靈菇; 液體培養; 種子制備; 栽培
【英文關鍵詞】 Pleurotus nebrodensis; Submerged fermentation; Spawn preparation; Cultivation
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2005年01期 【DOI】CNKI:SUN:SYJB.0.2005-01-009