CHEN Zhi-lan 1*; ZHANG Fu-ping 2; ZHONG Guo-hui 1; XIONG Wei 3; TIAN Yi-fa 1; ZHONG Zheng-chang 1; YANG Shuang 1 1Biology Center; Tibet Agriculture and Animal Husbandry College; 2Agriculture Department; Tibet Agriculture and Animal Husbandry College; 3Public Foundation Department; Tibet Agriculture and Animal Husbandry College; Linzhi; Tibet 860000; China
【英文摘要】 The nature of the starting material (i.e. powdered versus chipped fruit bodies of Hygrophorus russula) and increasing extraction frequencies were compared using polysaccharide yields as an index of extraction efficiency. Higher polysaccharide yields were obtained when extractions were carried out using powdered H. russula fruit bodies compared with fruit body chips, and crude polysaccharide yields of almost 4.3% were obtained from powdered material after two consecutive extractions using distilled water. Application of orthogonal testing to optimize extraction conditions revealed that polysaccharide yields were influenced mainly by the ethanol concentration used for precipitation, followed by temperature, solid:liquid ratio and treatment time. The optimal conditions were: extraction time 4 h, temperature 80 ℃, solid:liquid ratio 1:10, and ethanol concentration 85%. Under these conditions, a polysaccharide yield of 6.52% was obtained.
【英文關鍵詞】 Hygrophorus russula; Fruit body; Polysaccharide extraction
【基金】Sponsored by the Foundation of the Tibet Science and Technology Office(200568)
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2006年04期 【DOI】CNKI:SUN:SYJB.0.2006-04-013