竹文坤; 賀新生; 王茂
西南科技大學生命科學與工程學院; 西南科技大學生命科學與工程學院; 西南科技大學生命科學與工程學院 四川綿陽621002; 四川綿陽621002; 四川綿陽621002
【中文摘要】 研究了C源、N源、pH、溫度和料水比及不同培養基對勺狀亞側耳(Hohenbuehelia petaloides)Hp.831菌絲生長的影響。試驗結果表明,該菌絲生長的最適溫度、pH、C源、N源和含水量分別為25～30℃、6～7、羧甲基纖維素鈉、蛋白胨和60%～70%;馬鈴薯和松針的熱水提取液可促進該菌菌絲生長;該菌菌絲在配方7培養料上長勢最好,菌絲生長動態符合logistic方程:Y=109.6/(1+60.1131e-0.1894x)(y=菌絲生長長度mm,x=培養時間d)。
【英文摘要】 Mycelial growth rates of Hohenbuehelia petaloides, strain Hp831, were highest on media containing sodium carboxymethylcellulose (sodium CM-cellulose) and peptone, respectively as the major carbon and nitrogen sources, and with a water content of 60%-70%. The optimal pH range and temperature for mycelial growth were pH 6-7 and 25 ℃. Hot water extracts of potato and pine needles also promoted mycelial growth, the former most effectively. Among ten formulations tested, a composted substrate consisting of 50% cotton hulls and 50% sawdust was most effective in promoting H. petaloides mycelial growth; on this substrate, the dynamic relationship between mycelial growth and culture time accorded with the logistical equation: Y = 109.6/ (1+60.1131 e-0.1894x).
【中文關鍵詞】 培養特性; 勺狀亞側耳; Logistic方程
【英文關鍵詞】 Culture parameters; Hohenbuehelia petaloides; Logistic equation
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2007年01期 【DOI】CNKI:SUN:SYJB.0.2007-01-012